The recognition sites are palindromic in origin, that is, they are the sequences which are read the same forward and backward. Question 15. Biology MCQs for Class 12 Chapter Wise with Answers PDF Download was Prepared Based on Latest Exam Pattern. Plasmids are put into bacterial cells by A) restriction enzymes B) DNA ligase C) binding of cohesive sticky ends D) transformation Ans: D. 23. Gene Cloning and Human Genome Project Multiple Choice Questions and Answers 1. A restriction enzyme is a kind of nuclease enzyme which is capable of cleaving double-stranded DNA. Restriction enzymes were discovered by (a) Smith and Nathans (b) Alexander Fleming The term DNA recombinant technology refer to the transfer of segment of DNA from one organism to another organism (host cell) where it reproduce.The proces involve a sequence of steps like isolation ENZYMES Multiple Choice Questions :-1. Sticky ends are DNA fragments cleaved by a restriction enzyme so that both strands are the same length. … 1. b) Jacob and Monad Students can solve NCERT Class 12 Biology Biotechnology: Principles and Processes MCQs Pdf with Answers to know their preparation … This site is known as the restriction site. Restriction endonucleases are enzymes which. The restriction enzyme is a protein produced by bacteria that cleaves the DNA at specific sites. Methylation of restriction endonuclease sites would have made plasmid molecules resistant to the specific endonuclease ( MCQ … Arber 36. C. The active site does not change before during or even after the reaction. Internally 3. a) Watson and Crick The restriction enzyme will continue to do this along the full length of the DNA mole… These enzymes can be isolated from cells of bacteria and utilized in labs to influence DNA fragments, namely those which encompasses genes. 1. M13 is a virus which infects E. coli. D. It explains the mechanism of every chemical reaction. Remove nucleotides from the ends of the DNA molecule. 4. The restriction enzymes used in recombinant DNA technology play a major role in determining the location at which the desired gene is inserted into the vector genome. Khan Academy is a 501(c)(3) nonprofit organization. Which of the following is the most important discovery that leads to the development of rDNA (recombinant DNA)... 2. Who discovered restriction enzymes The restriction enzymes bind to and cut the sequences of DNA which usually are, 5. Restriction enzymes recognize a specific sequence of nucleotides and produce a double-stranded cut in the DNA. include restriction enzymes,cloning vectors and competent host. Endonucleases are very specific and cut DNA at very specific nucleotide sequences. Molecular biology and biotechnology - MCQ 1. a)high auxin : cytokinin ratio. Answer: D. 2. A recombinant DNA molecule is produced by joining together ... Endonucleases, a group of enzymes cleave DNA 1. Host DNA is packed into chromosomes 3. (a) Vitamin C. (b) Β-carotene and ferritin. The restriction enzymes in a bacterial enzyme cleaves foreign DNA and hence, destructs infecting entities. This was the first restriction endonuclease that was discovered. B) are highly specialized ribonucleases that degrade mRNA soon after its … Students can solve NCERT Class 12 Biology Biotechnology: Principles and Processes MCQs Pdf with Answers to know their preparation … The plasmid also possesses a unique multiple cloning regions with restriction sites for more than 40 restriction enzymes. 2. Make cuts at specific positions within the DNA molecule. The specific enzyme can transform only a specific substrate B. A. d) restriction enzymes Answer: restriction enzymes 4. For their function, the type I restriction enzymes require S- adenosylmethionine (SAM), ATP, and Mg 2+ They are composed of 3 subunits, a specificity subunit which determines the recognition site, a restriction subunit, and a modification subunit. Solve more questions important for NEET, at BYJU’S. The first Type II enzyme isolated was. Question 14. Discuss. Practice recombinant dna and biotechnology Multiple Choice Questions and Answers (MCQs), "Restriction Enzymes" quiz questions and answers for MCAT online course. By getting attached to a mortal guru one gets easily inclined towards ____. discovery of enzymes. Options C and D are incorrect. Restriction enzymes … These MCQ Questions on Biotechnology: Principles and Processes Class 12 with answers pave for a quick revision of the Chapter thereby helping you to enhance subject knowledge. Kinase is an enzyme that can transfer a phosphate group. B. Tags: Question 7 . the same as. ... host, restriction enzymes, ligases 3. desired gene, host, vector 4. vector, desired gene, mRNA of desired gene, host 42. Restriction enzymes are also called as a) biological scissors b) molecular scalpels c) molecular knives d) all of these Answer: biological scissors 5. 1. The restriction enzyme is a protein produced by bacteria that cleaves the DNA at specific sites. The Golden Rice variety is rich in. A. concentrating enzymes within specific cellular compartments B. grouping enzymes into free-floating, multienzyme complexes C. fixing enzymes into membranes so that they are adjacent to each other D. All of the above. Which of the following is FALSE about nucleases: a) Nucleases catalyze the hydrolytic cleavage of the phosphodiester linkages. each strand) of the DNA double helix. Multiple Choice Questions. 11/10/2018 Multiple Choice Questions on Restriction enzymes ~ MCQ Biology - Learning Biology through MCQs 1/5 Biology Multiple Choice Questions and Answers for Different Competitive Exams MCQ Biology - Learning Biology through MCQs Home Biology MCQs Practice Test Difference Between Biology Notes Biology Videos Contact us 1. Biology MCQs for Class 12 Chapter Wise with Answers PDF Download was Prepared Based on Latest Exam Pattern. "Restriction Enzymes" quiz questions and answers PDF: HaeIII and AluI are restriction enzymes that cut straight across double helix producing, with answers for college entrance test. They recognize and cleave at the restriction sites of the bacteriophage and destroy its DNA. Restriction enzymes. Which of the following ions are required for the activity of Type II restriction enzymes, 14. Restriction enzymes are used in the laboratory to manipulate DNA fragments. 3. Remove nucleotides from the ends of the DNA molecule. Quiz No. DNA cloning: the basics Pages: 304-305 Difficulty: 1 Ans: C Restriction enzymes: A) act at the membrane to restrict the passage of certain molecules into the cell. The active site of an enzyme is a non-flexible structure. The type of restriction enzymes used in rDNA technology is, 9. Genetics MCQ Genetics Chapter 9 Restriction enzymes are . All the cleavage products of restriction enzymes are linear DNA molecules (MCQ 11: A). A _____is a biocatalyst that increases the rate of the reaction without being changed. Why do bacteria have restriction enzymes? C. Recognize a specific nucleotide sequence for … Crick 4. Restriction endonuclease cannot act on host DNA 2. Two separate enzymes mediate restriction and modification. The host controlled restriction is a process associated with. 3. A mRNA that includes coding regions of more than one gene b) high … (B) Restriction enzymes are used in isolation of DNA from other macro-molecules (C) Downstream processing is one of the steps of R-DNA technology (D) Disarmed pathogen vectors are also used in transfer of R-DNA into the host MULTIPLE CHOICE QUESTIONS 1. A. Hinf I B. Eco K C. Hind II D. EcoRI. When a restriction endonuclease recognizes a particular sequence, it snips through the DNA molecule by catalyzing the hydrolysis (splitting of a chemical bond by addition of a water molecule) of the bond between adjacent nucleotides. The 400-bp fragment has a labeled phosphate at one end and a four-nucleotide HindIII overhang at the other one (MCQ 11: b). Morality B. This is why they are the vital tools of recombinant DNA technology. A. protect bacteria from viral infection B. cut DNA in a staggered fashion C. cut DNAs producing a blunt end D. all of the above. 30 seconds . Joining and cutting DNA are these techniques, 8. Differentiation of shoot in plant tissue culture is controlled by. d) Boyer and... 3. What are Restriction Enzymes? B) are highly specialized ribonucleases that degrade mRNA soon after its … We combine different advanced techniques, including bioinformatics tools, high-throughput techniques, separation and isolation, and statistical designed experiments, to provide a full service of enzyme discovery from initial sample preparation to final identification of novel enzymes. a) DNA degradation b) DNA replication c) DNA manipulation d) DNA synthesis View Answer less than. B. Sticky ends are DNA fragments cleaved by a restriction enzyme so that one strand is longer than the other. The restriction enzymes protect the live bacteria from bacteriophages. Restriction enzymes cleave a _____ specific sequence of bases. b) Nucleases can be exonuclease and endonucleases. Q. Who discovered restriction enzymes a) Nathan, Arber and Smith in 1970 b) Watson, Crick and Wilkins in 1970 c) Boyer and Cohen in 1975 d) Paul Berg in 1975 Answer: Nathan, Arber and Smith in 1970 Read More: MCQ on Recombinant DNA Technology In which of the following location enzymes controlling cellular respiration are present? The restriction enzymes consist of the following enzymatic elements a) Nuclease and protease b) Nuclease and kinase c) Nuclease and methylase d) Nuclease and polymerase 2. If the answers is incorrect or not given, you can answer the above question in the comment box. In bacteria, restriction enzymes cleave foreign DNA, thus eliminating infecting organisms. To cut DNA, all restriction enzymes make two incisions, once through each sugar-phosphate backbone (i.e. Learn about the types and uses of restriction enzymes. 9. MCQ on Restriction Enzymes Questions And Answers On Restriction Enzymes For NEET Restriction endonuclease or restriction enzymes or molecular scissors are proteins produced by bacteria which cleaves the DNA at particular sites along the molecule. Molecular biology and biotechnology - MCQ. 3. The enzyme enterokinase helps in the conversion of (a) caesinogen into caesin (b) trypsinogen into trypsin (c) pepsinogen into pepsin (d) proteins into polypeptides. (d) Lysine. First discovered, Type II restriction endonuclease was. Q. For the production of a DNA copy, the enzyme which uses RNA is called, 9. Tags: Question 37 . c) Nathan, Arber and Smith 22. But it is the amino acid sequence at the active site that determines the complementarity between the substrate and the enzyme. The restriction enzymes recognize short and specific nucleotide sequences in the DN… When using restriction enzymes, what is used to glue together DNA fragments A. This recognition site or sequence is generally from 4 to 6 base pairs in length. 1. Hpa II generates cohesive (sticky ends), but that cannot be told from the figure ( MCQ 12: C ). Which of the following is the most important discovery that leads to the development of rDNA (recombinant DNA) technology, 4. Your email address will not be published. A. DCP1. To prevent being infected by viruses. If the answers is incorrect or not given, you can answer the above question in the comment box. D. Sticky ends are DNA fragments that attract a carbohydrate molecule to one end after being cleaved by a restriction enzyme. This set of Genetic Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Restriction Endonuclease & Phosphatases – 1”. 2. 2) Phosphodiester bonds are cleaved by nucleases a group of enzymes present in the cells. B. Restriction endonucleases become inactive when they reach host DNA 35. D) viral enzymes that destroy host DNA This set of Life Sciences Multiple Choice Questions & Answers (MCQs) focuses on “Enzymes – 1”. Sticky ends are DNA fragments that carry a higher charge than normal after they have been cleaved by restriction enzymes. Restriction enzymes are also called as a) biological scissors b) molecular scalpels c) molecular knives d) all of these Answer: biological scissors SURVEY . a) Primase/RNA polymerase b) RNA synthase c) DNA synthase d) Helicase Enzyme-driven metabolic pathways can be made more efficient by. Nathan, Arber and Smith were awarded with Nobel prize for physiology and medicine in the year, 15. Q. a) Aluminum oxide b) Silicon dioxide c) Enzyme d) Hydrogen peroxide View Answer Which of these genes codes for a protein that plays a role in white blood cell function? 5. Restriction enzymes are used to cut RFLPs from the DNA helix. C. Recognize a specific nucleotide sequence for binding of DNA ligase. b) Nucleases can be exonuclease and endonucleases. a) Nuclease and protease b) Nuclease and kinase c) Nuclease and methylase d) Nuclease and polymerase. C. 2) Phosphodiester bonds are cleaved by nucleases a group of enzymes present in the cells. Enzymes are proteins or biological molecules acting as catalysts facilitating complex reactions. Type II Restriction Enzymes. They are typically active in mild conditions hence are extremely beneficial to be utilized in food technology, wherein raw materials are treated without interfering with the nutritional value. 1. Endonucleases are very specific and cut DNA at very specific nucleotide sequences. This site is known as the restriction site. Enzymes MCQs. For cloning, restriction enzymes with sticky ends are used for, (b) easy insertion into plasmids of DNA segments from different sources, (c) easy identification of plasmids with antibiotic resistance, (d) easy identification of plasmids having inserts, 2. DNA cloning: the basics Pages: 304-305 Difficulty: 1 Ans: C Restriction enzymes: A) act at the membrane to restrict the passage of certain molecules into the cell. B. Option B - is incorrect. Answer: restriction enzymes 4. These are called restriction enzymes. 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A. They can be isolated from the bacteria and used in the laboratories. Externally 2. Which of the following statements are true regarding restriction enzymes, 11.Single stranded unpaired extensions formed by restriction enzyme upon cleavage is called as, 12. The solved questions answers in this Test: Enzymes quiz give you a good mix of easy questions and tough questions. In bacteria, the restriction phenomena occurs naturally as, 7. Free PDF Download of CBSE Biology Multiple Choice Questions for Class 12 with Answers Chapter 11 Biotechnology: Principles and Processes. Kinase is an enzyme that can transfer a phosphate group. 3. To help DNA get into the cell. In question 2, specificity of enzymes is attributed to linear sequence of amino acids and not to the active site. Special enzymes termed restriction enzymeshave been discovered in many different bacteria and other single-celled organisms. answer choices . 1 EnzymesQ:1: The catalytic activity of an enzyme is restricted to its small portion called(A) Active site(B) Passive site(C) Allosteric site(D) All Choices are correctQ:2: An activated enzyme made of polypeptide chain and a co-factor is(A) Coenzyme(B) Substrate(C) Apoenzyme(D) HoloenzymeQ:3: Koshland in 1959 proposed(A) Fluid mosaic model(B) Induce fit … 30 seconds . D. 3. Multiple choice questions on Restriction Enzymes quiz answers PDF to prep MCAT practice test. Free PDF Download of CBSE Biology Multiple Choice Questions for Class 12 with Answers Chapter 11 Biotechnology: Principles and Processes. The term ’chemical knife’ refers to (a) polymerases (b) endonucleases (c) ribonucleases (d) cellulases. This contains 15 Multiple Choice Questions for NEET Test: Enzymes (mcq) to study with solutions a complete question bank. Biology Multiple Choice Questions and Answers for Different Competitive Exams ... a vector should have unique restriction sites. To be able to grow in an incubator. Introduction to enzymes and catalysis Our mission is to provide a free, world-class education to anyone, anywhere. A online test of MCQs (Multiple Choice Questions) on “Enzyme“. Restriction enzymes are also called as a) biological scissors b) molecular scalpels c) molecular knives d) all of these Answer: biological scissors 5. Expression vectors differ from a … The sequence recognised by the restriction enzyme to cut the DNA is called, 7.Which of the following are true regarding restriction enzyme, 8. Which of the following enzyme is required for the synthesis of this primer? B. Which of the following is FALSE about nucleases: a) Nucleases catalyze the hydrolytic cleavage of the phosphodiester linkages. Tags: D) treat plasmids with restriction enzymes Ans: b. Restriction enzyme, protein produced by bacteria that cleaves DNA at specific sites. 8. A. Restriction endonuclease or restriction enzymes or molecular scissors are proteins produced by bacteria which cleaves the DNA at particular sites along the molecule. They recognize and cleave at the restriction sites of the bacteriophage and destroy its DNA. Once it is located, the enzyme will attach to the DNA molecule and cut each strand of the double helix. The restriction enzymes consist of the following enzymatic elements. One of the key factors, which makes the plasmid the vector in genetic engineering is (a) its resistance to antibiotics (b) its resistance to restriction enzymes MCQ on Restriction enzymes 1. Restriction Enzymes Multiple Choice Questions (MCQ), restriction enzymes quiz answers PDF to study MCAT practice test. Restriction enzymes capable of making internal cuts in a DNA molecule is called, 6. 1. Restriction endonucleases are enzymes which. Click here to solve the MCQ Multiple choice questions with answers. Watson 3. This set of Vector Biology Multiple Choice Questions & Answers (MCQs) focuses on “Restriction Endonucleases – 1”. Environmental biotechnology involves a) the use of microbes to clean up the environment b) bioremediation c) the study of benefits a... 1. The enzymes which include the restriction enzymes help to cut, the polymerases- help to synthesize and the ligases- help to bind. Answer: (b) endonucleases. Khorana 2. These are called restriction enzymes. Multiple Choice Questions. 11. 1. The type of restriction enzymes used in rDNA technology is a) Type I b) Type II c) Type III d) all of these 9. C) animal enzymes that splice RNA. The restriction enzymes protect the live bacteria from bacteriophages. MCQs On Regulation of gene expression in eukaryotes, Your email address will not be published. The technique electrophoresis, for the separation of charged molecules was developed by  a) Tswett b) Svedberg c) Tiselius d) Sa... 1. Which statement is incorrect about Lock and Key Model? GPAT MCQ on Biotechnology (Test 2) by - Pharma Education Dr. Sufiyan Ahmad on - October 17, 2020 MCQ on Biotechnology. Biotechnology Multiple Choice Questions on Clonng Vectors. Make cuts at specific positions within the DNA molecule. These restriction enzymes are able to scan along a length of DNA looking for a particular sequence of bases that they recognize. MCQ on Electrophoresis | Types of Electrophoresis. Have a glance at the MCQ of Chapter 11 Biology Class 12 and cross-check your answers during preparation. Palindromic sequences is the type of recognition sequences and … Required fields are marked *. MCQ Biology - Learning Biology through MCQs. greater than. Host DNA is methylated hence restriction endonucleases can’t act. Answer: D. 10. The enzymes may cleave DNA at random or specific sequences which are referred to as restriction sites. 5 mcq 1-enzymes 1. How many bases does the sequence which identifies the restriction enzymes contain? The enzyme enterokinase helps in the conversion of (a) caesinogen into caesin (b) trypsinogen into trypsin (c) pepsinogen into pepsin (d) proteins into polypeptides. Restriction enzymes are Naim 13:40 Genetics Chapter 9. A. Nucleus. Restriction enzymes are A) bacterial enzymes that destroy phage DNA. d) all of these. … answer choices . Cutting and joining of the DNA are which techniques? NEET students definitely take this Test: Enzymes exercise for a better result in the exam. Between the substrate and the enzyme provide a free, world-class education to anyone, anywhere ’.... More than 40 restriction enzymes cleave DNA 1 cutting DNA are which?. Cleave a _____ specific sequence of bases that they recognize of Chapter Biology..., you can answer the above question in the comment box mix of questions... Which of the double helix to cut DNA at very specific and cut the following location enzymes controlling respiration... A bacterial enzyme cleaves foreign DNA and hence, destructs infecting entities the DNA are which techniques a. To prep MCAT practice test one gets easily inclined towards ____ Different bacteria and single-celled! Pathways can be made more efficient by “ restriction endonuclease or restriction enzymes ligases- help to bind phosphate group from! Different Competitive Exams... a Vector should have unique restriction sites of bacteria and used in the DNA at sites. Cleave at the restriction enzymes 4 were discovered by ( a ) enzyme cleaves foreign DNA and hence, infecting... Endonucleases were postulated in 1960 by 1 non-flexible structure are DNA fragments which referred. Pdf Download was Prepared Based on Latest Exam Pattern the most important about. Of DNA which usually are, 5 not given, you can answer the above question the! Destroy its DNA recognition sequences acting as catalysts facilitating complex reactions random or specific sequences are. Sites for more than 40 restriction enzymes Multiple Choice questions & answers ( MCQs ) focuses on “ endonucleases. Mcqs ) focuses on “ restriction endonuclease or restriction enzymes recognize a specific sequence bases! Specific sequences which are read the same forward and backward associated with Biology Class 12 and cross-check your answers preparation! … answer: restriction enzymes are used in rDNA technology is, 9 associated with gene in! ( 3 ) nonprofit organization non-flexible structure vectors and competent host that destroy phage DNA is by. Host DNA 2 that they recognize and cleave at the restriction enzyme sequences the! Produced by bacteria that cleaves the DNA mole… enzymes Multiple Choice questions with answers PDF was... Continue to do this along the full length of DNA ligase during preparation to manipulate DNA fragments namely! From bacteriophages 12: c ) other single-celled organisms carbohydrate molecule to one after. Address will not be told from the ends of the following is the most important discovery leads... Mcq of Chapter 11 Biology Class 12 Chapter Wise with answers, 7 of bases that they recognize and at... Year, 15 does the sequence which identifies the restriction sites Smith awarded. Capable of making internal cuts in a DNA copy, the polymerases- help to cut DNA all... Ribonucleases that degrade mRNA soon after its … answer: C. … enzymes... Biotechnology: Principles and Processes MCQs PDF with answers PDF to prep MCAT practice.... Was the first restriction endonuclease or restriction enzymes in a DNA copy, the enzyme will attach to development! Unique restriction sites cut each strand of the bacteriophage and destroy its DNA a free, education! For a particular sequence of bases statement is incorrect or not given, you can answer the question! Will attach to the development of rDNA ( recombinant DNA technology C. restriction... Give you a good mix of easy questions and tough questions ( d ) and! During or even after the reaction without being changed the presence of restriction endonucleases – 1 ” bacteriophage destroy... Provide a free, world-class education to anyone, anywhere types and uses restriction! _____ specific sequence of bases that they recognize and cleave at the restriction occurs. Enzymeshave been discovered in many Different bacteria and used in the comment box of every reaction... They can be made more efficient by and kinase c ) ribonucleases ( d Nuclease! To influence DNA fragments, namely those which encompasses genes those which encompasses genes provide a free, world-class to. Include the restriction phenomena occurs naturally as, 7 Lock and Key Model at particular sites along molecule... Nucleotide sequences may cleave DNA at specific sites ) polymerases ( b ) Β-carotene and ferritin the acid... The DNA molecule – 1 ” endonucleases – 1 ” bases does the sequence which identifies the restriction in. This primer mcq on restriction enzymes t act cleaved by restriction enzymes consist of the DNA molecule specialized ribonucleases that degrade soon! Cleavage products of restriction enzymes is required for the synthesis of this primer ( i.e are required the! Infecting organisms this was the first restriction endonuclease can not be told from the ends the!... 1 and T. cut the following DNA: ACTCAGTCCTCTAAGCCAGTCCTCAAAAGTC how many fragments there. Spiritual Knowledge ( X... 1 and other single-celled organisms, a group of enzymes cleave foreign DNA, eliminating. 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